Herbal Advantage, Inc.
"Informed Cholesterol Management"


Please see related story: A Personal Awakening
Read information about Linus Pauling
Information about Vitamin C
A Lipoprotein Risk Assessment Test for Informed Cholesterol Management
The Benefits of the VAP Test Comprehensive lipoprotein analysis
Who is a candidate for a VAP Test? Risk Factors
RETURN TO HEART TECHNOLOGY PAGE

The Vertical Auto Profile, or VAP, Test is a Comprehensive Lipoprotein Risk Assessment Test for Total Cholesterol/Lipid Management. The VAP Test directly measures risk factors associated with heart disease, providing more accurate information than the typical cholesterol test which calculates the LDL value. Additionally, the VAP test directly measures other cholesterol / lipoproteins that are now recognized as independent risk factors. The VAP Test is not only more accurate, it is cost effective, and is currently reimbursed by over 600 insurance carriers, including Medicare.

The patented VAP Test separates lipoproteins according to density using density gradient ultra centrifugation. Plasma samples are placed in an ultracentrifuge tube and adjusted to a high density; a lower density solution is layered on top the plasma samples, and the tubes are placed in a vertical ultracentrifuge rotor. During centrifugation, the two density layers blend to form a continuous density gradient, and the lipoproteins present in the plasma sample float toward the top of the tube and stop at their characteristic density. Thus, VLDL will float to the top of the tube, LDL will form a band in the middle, and HDL will remain near the bottom of the tube. Use of the vertical rotor provides an optimal combination of resolution of individual lipoprotein families and speed of separation.

Following separation, the tubes are placed in a fractionator assembly and punctured through the bottom with a needle. The material in the tube is drained from the bottom where it is mixed with an enzymatic reagent. The enzymatic reagent reacts with the cholesterol in the sample to form a colored product, which can be detected and measured photometrically. Thus, a cholesterol profile is developed based on the amount of color formation from the bottom to the top of the tube.

The procedure measures "real LDL" and is not affected by high triglyceride levels. The use of the vertical rotor allows for a spin cycle of less than 60 minutes .Each rotor contains a control sample of known cholesterol content, as determined by the U.A.B Hospital Clinical Laboratory and the Northwest Lipid Research Laboratory ("NWLRL"), part of the National Cholesterol Reference Network coordinated by the Centers for Disease Control.

Total cholesterol in each sample is determined by comparison of the total area under the profile with the area of control. Sub curves corresponding to each lipoprotein are decomposed from the total profile using proprietary computer software, and the cholesterol content of each lipoprotein is again determined by measuring the area under the sub curve.


Please see related story: A Personal Awakening
Read information about Linus Pauling
Information about Vitamin C
A Lipoprotein Risk Assessment Test for Informed Cholesterol Management
The Benefits of the VAP Test Comprehensive lipoprotein analysis
Who is a candidate for a VAP Test? Risk Factors
RETURN TO HEART TECHNOLOGY PAGE


© 1997 - 2003 by Herbal Advantage, Inc.
131 Bobwhite Road, Rogersville, Missouri, 65742, USA
Telephone: 417-753-4000 or 800-753-9199, Fax: 417-753-2000
Last Updated: 08-17-02 by SJM